Circulating Human Neonatal Naïve B Cells are Deficient in CD73 Impairing Purine Salvage

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Circulating Human Neonatal Naïve B Cells are Deficient in CD73 Impairing Purine Salvage

BACKGROUND Extracellular purines, in particular adenosine (Ado) and adenosine-triphosphate, are critical immunoregulatory molecules. Expression and activity of purine ecto-enzymes on B cells in neonatal and adult blood may influence their function and has been incompletely characterized. METHODS Mononuclear cells were isolated from human neonatal (cord blood) or adult (peripheral blood) subje...

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CD73-deficient mice are resistant to carcinogenesis.

CD73 is a cell surface 5'-nucleotidase that converts AMP to adenosine, an immune suppressive molecule. CD73 may promote immune escape in cancer by contributing to the degradation of extracellular ATP released by dying cancer cells in hypoxic tumors or following chemotherapy. However, whether CD73 exerts a critical oncogenic function during tumorigenesis is unknown. In this study, we used geneti...

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Circulating human B lymphocytes are deficient in nucleotide excision repair and accumulate mutations upon proliferation.

Faithful repair of DNA lesions is a crucial task that dividing cells must actively perform to maintain genome integrity. Strikingly, nucleotide excision repair (NER), the most versatile DNA repair system, is specifically down-regulated in terminally differentiated cells. This prompted us to examine whether NER attenuation might be a common feature of all G₀-arrested cells, and in particular of ...

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Human blood IgM " memory " B cells are circulating splenic marginal zone B cells

The human peripheral B cell compartment displays a large population of IgM IgD CD27 memory B cell carrying a mutated Ig + + + “ ” receptor. We show here, by phenotypic analysis, CDR3 spectratyping during a T-independent response and gene expression profiling of the different blood and splenic B cell subsets, that blood IgM IgD CD27 cells correspond to circulating splenic marginal zone B cells. ...

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Bone marrow dendritic cells deficient for CD40 and IL-23p19 are tolerogenic in vitro

Objective(s): In addition to pro-inflammatory role, dendritic cells (DCs) can also be anti-inflammatory when they acquire tolerogenic phenotype. In this study we tested the role of CD40 and IL-23p19 in antigen presenting function of bone marrow-derived DCs (BMDCs) by comparing BMDCs derived from CD40 knockout (CD40KO-DCs) and IL-23p19 (IL-23p19KO-DCs) knockout mice wit...

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ژورنال

عنوان ژورنال: Frontiers in Immunology

سال: 2016

ISSN: 1664-3224

DOI: 10.3389/fimmu.2016.00121